Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Influence of adrenomedullin on the enzymatic activity of dipeptidyl peptidase 3 in vitro

doi: 10.1080/14756366.2026.2700037

Figure Lengend Snippet: Antibody-mediated reversal of peptide-induced inhibition of DPP3 activity. ADM (panel A), ADM1–14 (panel B), and ADM-Gly (panel C), respectively, was added at a constant final concentration of 10 µM in the DPP3 activity assay, and increasing amounts of the anti-N-terminal ADM antibody and the negative control antibody Palivizumab, respectively, were added at the indicated concentrations. The inhibition achieved with 10 µM of each peptide was defined as 100% inhibition, and inhibition measured in the presence of antibodies was related to this. The maximal reversal of peptide-induced inhibition of DPP3 activity was defined as 0% inhibition. Shown are mean (SD) values gained from duplicate measurements. For all three peptides, the reversal of peptide-induced inhibition of DPP3 activity by the anti-ADM antibody was statistically significant compared to the negative control antibody Palivizumab ( p > 0.05 by ANOVA).

Article Snippet: A humanised anti-N-terminal ADM monoclonal antibody (initially named Adrecizumab, later Enibarcimab) was purchased from (MedChemExpress, Monmouth Junction, USA).

Techniques: Inhibition, Activity Assay, Concentration Assay, Negative Control

Journal: International Dental Journal

Article Title: Chemokine-Like Receptor 1 Knockdown Suppresses Oral Squamous Cell Carcinoma Progression by Reducing Oxidative Phosphorylation

doi: 10.1016/j.identj.2026.109479

Figure Lengend Snippet: CMKLR1 promotes OSCC cell migration, invasion, and EMT. Representative images and quantification of wound healing assays in (A) Cal-27 cells with CMKLR1 knockdown and (B) SCC-9 cells with CMKLR1 overexpression. Transwell invasion assays and quantification in (C) Cal-27 and (D) SCC-9 cells, revealing a decreased and increased invasive capacity after CMKLR1 knockdown and overexpression, respectively. Scale bar = 100 μm. (E) Western blot analysis of EMT markers (E-cadherin, N-cadherin, vimentin, Snail, and Slug) in CMKLR1-modified OSCC cells. * P < .05; ** P < .01; *** P < .001.

Article Snippet: These membranes were then blocked with 5% blocking buffer in Tris-buffered saline with 0.1% Tween 20 for 1 hour at room temperature, with this followed by overnight incubation at 4 °C with primary antibodies against CMKLR1 (#ab306554; Abcam, Cambridge, UK), β-actin (# P30002 ; Abmart, Shanghai, China), E-cadherin (#610181; BD Biosciences, Shanghai, China), N-cadherin (#13116; Cell Signaling Technology, Danvers, MA, USA), vimentin (#5741; Cell Signaling Technology), Snail (#3879; Cell Signaling Technology), Slug (#9585; Cell Signaling Technology), PI3K (#AF6241; Affinity Biosciences, Cincinnati, OH, USA), PGC1 (#AF5395; Affinity Biosciences), Phospho-PI3K (#AF3241; Affinity Biosciences), and OXPHOS Cocktail (#PK30006; Proteintech, Rosemont, IL, USA).

Techniques: Migration, Knockdown, Over Expression, Western Blot, Modification